Operator assisted and unassisted usage rates can be provided upon request for companies and/or non-profit groups wishing to use the flow cytometry facility.
Copies of Flowjo software may be purchased for internal laboratories at the University of Toronto for a discounted rate (~375 CAD/license/year).
The University of Toronto’s Flow Cytometry Facility is pleased to announce a partnership with WORK-FLOW™ to bring a comprehensive (3 hours, 37 minutes, 4 seconds) online flow cytometry curriculum to its training program. All new registrants will be required to participate in the WORK-FLOW™ program regardless of previous experience or unless they can provide earlier WORK-FLOW™ certification.
There are a minimum of five requisite sections (average time/section, 30 minutes 34 seconds) to review with an optional sixth course (1 hour, 4 minutes, 16 seconds) if you plan to operate the BD FACSMelody™ for sorting. Each section has their own testing phases. The five core sections cover the following topics:
Details the process of generating data with emphasis on the integration of an instrument’s three main systems (fluidics, optics, and electronics) (Time, 00:31:24).
Explains the use of antibodies and their fluorescent conjugates in flow. Concepts such as spectral overlap and compensation are also discussed (Time, 00:34:45).
Explains the types of controls necessary for a successful flow cytometry experiment. Additional information on the sources of experimental variability are discussed along with methods for addressing them and standardizing experiments for consistency between runs. A separate presentation on compensation theory is also provided (Time, 00:27:37).
Reviews how data is displayed and interpreted, selecting populations for analysis via gating, and common data presentation formats for analysis and publication (Time, 00:29:06).
Reviews the differences between descriptive and inferential statistics along with standard student’s t-tests (Time, 00:29:56).
Provides an overview of the operational mechanics of a cell sorter. In this course you will learn: how to select nozzle size, sheath pressure, droplet breakoff, stream charge/deflection, collection, drop delay, acquisition/gating on your population of interest, conflicts, sorting, post-sort purity, recovery, and yield, sample standardization, temperature/pressure variables, collection media, and biosafety (Time, 01:04:16).
Within these five to six modules, WORK-FLOW™ is filled with a wealth of flow cytometry related information that clients will find very valuable and are encouraged to explore on their own.
Test results must be presented to the flow cytometry facility before instrument training can be scheduled.
The average cost of training varies between $550 – 750+, depending on experimental complexity. Please contact us to discuss your plans to receive an estimate of training costs for your application.
Please download our registration forms to become a facility member to gain access to the WORK-FLOW™ program and start the instrument training program.
Meet the friendly and diverse team that keeps the University of Toronto Flow Cytometry Facility running on a day-to-day basis.